ATP and ADP are energy molecules Which produced during light reaction and that is utilised in the dark reaction bye release of energy.
Production of ATP and ADP during Light Reaction:
Uses of ATP and NADPH - ATP and NADPH (Nicotinamide adenine dinucleotide phosphate) are have role same of Calvin cycle is almost same like krebs cycle as the products that produced during the light reaction enters the Calvin cycle as a carbon dioxide and release as glucose.
Here ATP are used as high energy molecules and in NADPH acts as reducing agents which provides electron to form glucose. Calvin cycle produces three carbon containing compounds glyceraldehyde 3-phosphate.
6 CO2 + 12 (NADPH + H+) + 18 ATP → C6H12O6 + 12 NADP+ + 18 ADP + 18 Pi + 6 molecules of water.
Calvin cycle is made up of three phase among them the middle one is the reduction. In this phase two step reaction take place by the hydrolysis of ATP and reduction of 3 phosphoglycerate to phosphoglyceraldehyde.
Phosphorylate 3 phosphoglycerate enzyme transfer phosphate from ATP molecules and causes the following reaction-
* Uses 6 molecules of ATP to produce six molecules of 1,3 bis phosphoglyceric acid.
12 PGA + 12 ATP- → 12 molecules of 1,3 di PGA + 12 ADP.
* It acts as primer of 1 ,3 phosphoglyceric acid and add electrons from NADPH.
* Electrons of NADPH reduce carboxyl group of 1,3 bis phosphoglycerate to 3 phosphoglyceralde.
12 molecules of 1,3 Di PGA + 12 NADPH2 - → 12 PGAlD + 12 NADP.
The third step of Calvin cycle is regeneration of the initial compounds that is ribulose bis phosphate-
This energy required three molecules of ATP and produces RuBP molecules that is produced and is available for start of the cycle again.
5 PGAlD (Phosphoglyceraldehyde Glyceraldehyde) → 5 molecules of DHAP.
For the net synthesis of three molecules of glucose 3phosphate, 9 molecules of ATP are required and 6 molecules of NADPH2 are required.
Raw material for Calvin cycle is glucose 3 phosphate that is used for synthesis of glucose and other carbohydrates. Here 18 molecules of ATP is required and 12 molecules of nicotinamide adenine dinucleotide phosphate (NADP) is required for production of one Glucose molecule.
Blackmans law of limiting factor- factors that affect the reaction is known as Black nails law of limiting factors. These are light intensity carbon dioxide concentration etc.